Johnstone earned his recall after a best on ground performance in the Lions Reserves' win against the Western Magpies last Sunday afternoon.
Johnstone replaces Matt Austin who has been omitted after playing his first three consecutive games with the Club.
The Lions will be keen to turn the tables on the Blues this Saturday night after Carlton ran out 19-point winners in their last encounter back in Round 2 at Etihad Stadium.
It promises to be a battle between two teams looking to solidify a position inside the AFL’s top eight. The Lions currently sit in 4th position with six wins while the Blues are 5th with five wins.
The full Lions team for Round 11 is as follows:
B: Ashley McGrath (9), Joel Macdonald (26), Jed Adcock (7)
HB: Sam Sheldon (3), Lachlan Henderson (14), Josh Drummond (39)
C: Scott Harding (5), Simon Black (20), Daniel Rich (10)
HF: Michael Rischitelli (35), Jonathan Brown (16), James Polkinghorne (31)
F: Rhan Hooper (33), Daniel Bradshaw (36), Luke Power (6)
Foll: Mitch Clark (1), Cheynee Stiller (32), Justin Sherman (11)
Int: Tim Notting (8), Albert Proud (2), Jared Brennan (17), Travis Johnstone (4)
Emerg: Matt Austin (37), Aaron Cornelius (44), Jack Redden (30)
IN: Travis Johnstone
OUT: Matt Austin (omit)